Desulfitobacterium: growth medium, add salt to taste! ;)

Preparing a growth medium is as esay as following a recipe! :)
This is the colture medium I'm going to use with Desulfitobacteria hafniense DCB-2, D. hafniense TCE-1, D. chlororespirans, D. dehalogenans, D. PCE1.

Pay attention to respect the right concentration and follow the protocol i've posted to prepare anaerobic solutions.

Starting solutions:

• M1= K2HPO4 (0.2 M)  
• M2= NaH2PO4 * 2H2O (0.2 M) 
• M3= Resazurine (0.5 g/L) 
• M4= EDTA 500 (mg/L) 
• M5=

• Vitamin H (50 mg/L) 
• p-aminobenzoate (250 mg/L) 
• Pantothenate (50 mg/L) 
• Folic acid (20 mg/L) 
• Lipoic acid (50 mg/L) 
• Vitamin B6 (100 mg/L) 
• Nicotinamide (550 mg/L) 
• M6= Vitamin B1 (100 mg/L)
• M7= Vitamin B2 (50 mg/L) 
• M8= Vitamin B12 (50 mg/L)
• M9= NH4HCO3 (114 mM) + NaHCO3 (906 mM)
• M10= Na2S * 9H2O (1M)
• M11= CaCl2 * H2O (30 mM) + MgCl2 * 6 H2O (20 mM)
• M12= Yeast extract (4%)
• M13= Na2SeO3 (6 mg/L)

All solutions were made in anaerobic water and flushed with N2 (except for M9 and M10 which contain volatile compounds).

Basic medium

• 21 ml M1 
• 7 ml M2 
• 1 ml M3
• 971 ml “sub-boiled” water

Cold solutions

• Cold A: in anaerobic, sterile bottle with 20 ml sterile demi-water added were: 1 ml M4, 1 ml M5, 1 ml M6, 1 ml M7, 1 ml M8, 0,5 ml M13. 
• Cold B: 1 ml M10, 49 ml M9 filter-sterilised. 
• Cold C: ~30 ml M11.

Completing the growing medium

To 18 ml basic medium (M1+M2+M3) add: 

• 1 ml Cold B 
• 0.5 ml Cold A
• 0.5 ml Cold C
• 1 ml 4% yeast extract
• Inoculum (10%)

Desulfitobacteria hafiense DCB-2 (University of California)

So calculators in hand and good luck!
Soon to your screen the Best Pasta Ever recipe! :D