Thesis - technical hitches :S

Beautiful folk! :)

Here I am with the first problems with my thesis...

I'm actually preparing the colture medium for my anaerobic bacteria, of course to be sure that no oxygen is inside i need a redox indicator. In my previous post i mentioned Resazurin, apparently this compound create problems to our HPLC-MS releasing crystals in the columns. I should find a way to take off the Resazurin before the analysis... not so easy considering that I'm monitoring PFCs (PFOS, PFOA and PFBS) and the structure of my redox indicator doesn't allow me to use a lot of extraction techniques without damaging my metabolites! :(

Checking also other redox indicators i could notice how they are really similar between them...

In conclusion i thought to use Titanium(III)-citrate that becomes white once oxydated. Of course i should take into account in my final calculations that it could degradate my PFCs as shown in the following article: http://www.mendeley.com/research/reductive-defluorination-of-perfluorooctane-sulfonate/# ...

I'm going to propose this to my supervisor and I'll let you know! :):):)

Besitooos!